Journal: Toxicological Sciences

Article Title: Protective Role of Surfactant Protein-D Against Lung Injury and Oxidative Stress Induced by Nitrogen Mustard

doi: 10.1093/toxsci/kfy188

Figure Lengend Snippet: Effects of loss of SP-D on NM-induced HO-1 expression. Histological sections, prepared 14 days after treatment of WT and SP-D−/− mice with PBS or NM, were stained with antibody to HO-1. Binding was visualized using a DAB peroxidase substrate kit. One representative section (staining intensity, 2) from 3 to 4 mice/treatment group is shown. Magnification, 60×; arrows, macrophages; arrowheads, Type II cells.

Article Snippet: This was followed by overnight incubation at 4°C with rabbit IgG or rabbit polyclonal anti-HO-1 (1:250; Enzo Life Sciences, Farmingdale, New York), anti-Ym-1 (1:75; Abcam, Cambridge, Massachusetts), anti-iNOS (1:500; Abcam), anti-COX-2 (1:800; Abcam), anti-MMP-9 (1:50, Santa Cruz Biotechnology, Dallas, Texas), anti-pro-SP-C (1:750, EMD Millipore, Billerica, Massachusetts), or anti-mannose receptor (MR)-1 (1:400; Abcam), antibodies.

Techniques: Expressing, Staining, Binding Assay

Journal: PLOS ONE

Article Title: HIF3A gene disruption causes abnormal alveoli structure and early neonatal death

doi: 10.1371/journal.pone.0300751

Figure Lengend Snippet: (A) Representative images of immunohistochemical staining of SCD1 in neonatal lungs. Scale bar indicates 50 μm. (B) Semiquantitative analysis of SCD1-positive cell counts. Ratios were calculated by dividing SCD1-positive cells from the total cell count. The results are presented as the mean ± SD. Comparisons were analyzed by Kruskal-Wallis test followed by Dunn test. (n = 5). *p < 0.05. (C) Fatty acid composition in lungs of neonatal mice at P0. Total lipids were extracted from lungs and analyzed by gas chromatography. The results are presented as the mean ± SD. All comparisons were analyzed by one-way ANOVA, Tukey’s post hoc test (n = 9 each). *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: For immunohistochemistry, deparaffinized sections were incubated overnight at 4°C with rabbit polyclonal anti Ki67 antibody (1:400) (NB500-170; Novus Biologicals, Centennial, CO, USA), rabbit polyclonal anti-PDPN antibody (1:1000) (ab11936; Abcam, Cambridge, UK), rabbit polyclonal anti-Uteroglobin/CC10 antibody (1:2000) (10490-1-AP; Protintech, Rosemont, IL, USA), rabbit polyclonal anti pro SP-C antibody (1:1000)(AB3786; Millipore, Darmstadt, Germany) and rabbit monoclonal anti-SCD1 antibody (1:100) (#2794; Cell Signaling Technology, Danvers, MA, USA).

Techniques: Immunohistochemical staining, Staining, Cell Counting, Gas Chromatography

Journal: Toxicological Sciences

Article Title: Protective Role of Surfactant Protein-D Against Lung Injury and Oxidative Stress Induced by Nitrogen Mustard

doi: 10.1093/toxsci/kfy188

Figure Lengend Snippet: Effects of loss of SP-D on NM-induced Ym-1 and MR-1 expression. Histological sections, prepared 14 days after treatment of WT and SP-D−/− mice with PBS or NM, were stained with antibody to Ym-1 (upper panels) or MR-1 (lower panels). Binding was visualized using a DAB peroxidase substrate kit. One representative section (Ym-1, staining intensity, 2; MR-1, staining intensity, 3) from 3 to 4 mice/treatment group is shown. Magnification, 60×; arrows, macrophages; arrowheads, Type II cells.

Article Snippet: This was followed by overnight incubation at 4°C with rabbit IgG or rabbit polyclonal anti-HO-1 (1:250; Enzo Life Sciences, Farmingdale, New York), anti-Ym-1 (1:75; Abcam, Cambridge, Massachusetts), anti-iNOS (1:500; Abcam), anti-COX-2 (1:800; Abcam), anti-MMP-9 (1:50, Santa Cruz Biotechnology, Dallas, Texas), anti-pro-SP-C (1:750, EMD Millipore, Billerica, Massachusetts), or anti-mannose receptor (MR)-1 (1:400; Abcam), antibodies.

Techniques: Expressing, Staining, Binding Assay